Developing of novelty co-cultured intestinal simulation system in vitro

The industries of prebiotics and probiotics have grown up and the research on bowel disease treatment and microbiota has been more attractive. However, most of those studies use animal model, and their equipment, time and labor cost increase dramatically due to more and more rigorous regulation on animal study. Hence, animal study cannot afford the requirement for developing novel beneficial gut bacteria. In addition, human intestinal pathophysiology is quite different from animal. In order to create novel and high economic human-like digestive systems, this work aims to develop simulated human intestinal microphysiological system to investigate the interaction between food component and microbiota in vitro. Our goal is to partially replace animal test on microbiota research with the novel system we developed and enable us to further study the interaction of intestinal epithelial cells and food component.